![]() ![]() ![]() The combination of dynamic light scattering with Raman spectroscopy characterizes a wealth of chemical, structural, and physical parameters of biotherapeutic proteins under formulation conditions - at high concentrations up to 100 mg/ml and using a wide range of buffers and excipients. Raman spectroscopy delivers information on protein unfolding by monitoring the variations in molecular vibrations that result from changes in secondary and tertiary protein structure. ![]() Such detailed information supports both the effective application of Quality by Design and the efficient development of biosimilars. The combination of DLS and Raman spectroscopy allows measurement of protein size and structure from a single small volume sample, providing unique insight into protein folding, unfolding, aggregation, agglomeration and oligomerization. Raman spectroscopy enables monitoring of the changes in secondary and tertiary protein structure. This new system combines industry-leading Zetasizer dynamic light scattering (DLS) technology, for sizing of proteins and other biomolecules, with Raman spectroscopy. The new Zetasizer Helix from Malvern Instruments is a powerful tool for early stage biopharmaceutical development that enables the detailed study of mechanisms of protein aggregation. ![]()
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